Journal: PLoS ONE

Article Title: Imaging Neuroinflammation In Vivo in a Neuropathic Pain Rat Model with Near-Infrared Fluorescence and 19 F Magnetic Resonance

doi: 10.1371/journal.pone.0090589

Figure Lengend Snippet: A , B show the affected right sciatic nerve from the CCI leg shown in stained with anti-CD68 antibody to reveal the presence of macrophages infiltrating the nerve. C , D CD68 positive cells are not present in the left leg (contralateral to surgery ) of the same CCI animal. E , F show a nerve from a separate CCI animal that also exhibits infiltration of CD68 positive cells. The boxed area is enlarged to reveal the granular cytoplasm (black arrow) of the macrophages, indicative of the presence of the nanoemulsion. Sham surgical sciatic nerve and non-surgical control sciatic nerve do not exhibit any CD68 positive cells ( G , H ) and ( I , J ). The fluorescent images A, C, G, and I were all acquired at the same sitting with the exact same image acquisition parameters. Macrophages grown in cell culture take up the nanoemulsion, exhibited as particles evident by both confocal fluorescence emissions 700–850 nm of NIR label (DiR) ( K , M ) as well as transmitted light ( L , N ).

Article Snippet: In separate immunohistochemical experiments using comparable protocols, the recovered control, sham and CCI sciatic nerves were prepared for examination using mouse anti rat CD68 antibody (MCA341R, AbD Serotech, Raleigh, NC) and Alexa fluor 488 donkey anti mouse secondary antibody (A-21202, Invitrogen, Carlsbad, CA) to assess the presence of macrophages that infiltrate the nerve.

Techniques: Staining, Control, Cell Culture, Fluorescence

Journal: Stem Cell Research & Therapy

Article Title: Poly(ethylene glycol)-modified silk fibroin membrane as a carrier for limbal epithelial stem cell transplantation in a rabbit LSCD model

doi: 10.1186/s13287-017-0707-y

Figure Lengend Snippet: Corneal epithelial regeneration and repair after LESC/SF graft transplantation. a Rabbit corneas 4 months after transplantation (left panels, corneal epithelial cells marker CK12 staining; middle panels, enlarged pictures of the framed area; right panels, proposed LESC marker ABCB5 staining in the limbus). The four groups are as follows: (1) cornea from normal eyes (positive control), (2) cornea from LSCD model (no grafts, negative control), (3) PEG-modified SF membrane transplantation (SF grafts), and (4) LESCs cultured on PEG-modified SF membrane transplantation (LESC/SF grafts). Arrows point to the ABCB5 + LESCs in the limbus. b Macrophage antigen CD68 (located at endosome, lysosome, and cell membrane) staining showed inhibited inflammation in the corneas 4 months after LESC/SF graft transplantations. Arrows point to CD68 + macrophages in the corneas. c Corneal epithelial thickness in the four groups. d Corneal stromal thickness in the four groups. e Area percentages of CK12 + epithelium in total corneal epithelium in the four groups. Transplantation experiments were performed in three rabbits per group. Three consecutive cross-sections from three rabbits per group were analyzed. Data was shown as mean ± SD. One-way ANOVA: * P < 0.05; ** P < 0.01; n.s. not significant. Scale bar, 50 μm. ABCB5 sub-family B, member 5, CK12 cytokeratin12, DAPI 4′,6-diamidino-2-phenylindole, LESC limbal epithelial stem cell, SF silk fibroin

Article Snippet: The following antibodies were used: rabbit anti-p63α monoclonal antibody (ab124762; Abcam), mouse anti-ABCB5 monoclonal antibody (ab140667; Abcam), rabbit anti-α-SMA polyclonal antibody (BM0002; Boster), mouse anti-Ki67 monoclonal antibody (550609; BD BioSciences), goat anti-CK12 polyclonal antibody (sc-17098; Santa Cruz), rabbit anti-CK12 monoclonal antibody (ab185627; Abcam), mouse anti-CK7 antibody (ab9021; Abcam), mouse anti-BrdU monoclonal antibody (ab1893; Abcam), rabbit anti-Claudin-1 polyclonal antibody (ab15098; Abcam), mouse anti-CD68 monoclonal antibody (MCA341R; AbD Serotec), and mouse anti-CD31 monoclonal antibody (ab9498; Abcam).

Techniques: Transplantation Assay, Marker, Staining, Positive Control, Negative Control, Modification, Membrane, Cell Culture